Imaging Flow Cytometry: Methods And Protocols Official

: Beyond fluorescence, IFC provides data on cell size, shape, circularity, and internal complexity.

: Non-specific binding is prevented using anti-Fc antibodies. Cells are then stained with fluorophore-conjugated antibodies, viability dyes, or nuclear dyes like DAPI or Hoechst to provide spatial landmarks. Imaging Flow Cytometry: Methods and Protocols

: Cells must be in a single-cell suspension to avoid clogging the thin fluidic channels used for imaging. : Beyond fluorescence, IFC provides data on cell

While IFC shares many preparatory steps with conventional flow cytometry, it requires specific optimizations to ensure high-quality imagery. : Beyond fluorescence